Current Biotechnology

Pabulo Henrique Rampelotto  
Center of Biotechnology and PPGBCM
Federal University of Rio Grande do Sul
Porto Alegre, RS


Industrial Production of the Cell Protectant Ectoine: Protection Mechanisms, Processes, and Products

Author(s): Hans Jorg Kunte, Georg Lentzen and Erwin A. Galinski

Affiliation: Federal Institute for Materials Research and Testing (BAM), Division FB 4.1 Biodeterioration and Reference Organisms, Unter den Eichen 87, 12205 Berlin, Germany.

Keywords: Bacterial milking, batch fermentation, continuous culture, ectoine excretion, Hofmeister effect, kosmotrope, “leaky” mutant, osmophobic effect, protein protection, preferential exclusion.


Bacteria, Archaea and Eukarya can adapt to saline environments by accumulating compatible solutes in order to maintain an osmotic equilibrium. Compatible solutes are of diverse chemical structure (sugars, polyols, amino acid derivatives) and are beneficial for bacterial cells not only as osmoregulatory solutes, but also as protectants of proteins by mitigating detrimental effects of freezing, drying and high temperatures. The aspartate derivative ectoine is a wide spread compatible solute in Bacteria and possesses additional protective properties compared with other compatible solutes, and stabilizes even whole cells against stresses such as UV radiation or cytotoxins. The protective properties of ectoine for proteins can be explained by its strong (kosmotropic) interaction with water and subsequent exclusion from protein surface, the decrease of the solubility of the peptide backbone and the strengthening of intramolecular hydrogen bonds (secondary structures). The stabilizing and UV-protective properties of ectoine attracted industry, which saw the potential to market ectoine as a novel active component in health care products and cosmetics. In joint efforts of industry and research large-scale fermentation procedures have been developed with the halophilic bacterium Halomonas elongata used as a producer strain. The two key technologies that allow for the annual production of ectoine on a scale of tons are the bacterial milking procedure and the development and application of ectoine-excreting mutants (“leaky” mutant). The details of these two procedures including the strain development and fermentation processes will be introduced and current and future applications of ectoine will be discussed.

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Article Details

Page: [10 - 25]
Pages: 16
DOI: 10.2174/22115501113026660037