Recent Patents on Biotechnology

Rongling Wu
Departments of Public Health Sciences and Statistics
Pennsylvania State College of Medicine
Hershey, PA
USA

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Mini-Review: Recent Developments in Hydroxynitrile Lyases for Industrial Biotechnology

Author(s): Elisa Lanfranchi, Kerstin Steiner, Anton Glieder, Ivan Hajnal, Roger A. Sheldon, Sander van Pelt and Margit Winkler

Affiliation: ACIB GmbH (Austrian Centre of Industrial Biotechnology GmbH), Petersgasse 14/III, A-8010 Graz, Austria.

Keywords: Asymmetric synthesis, biocatalysis, biotransformation, cascade reactions, C-C coupling, chiral building blocks, cyanogenesis, cyanohydrin, enantioselectivity, enzyme engineering, HNL, hydrocyanic acid, hydroxynitrile lyase, industrial process, mandelonitrile lyase, plant enzymes, oxynitrilase, stereoselective.

Abstract:

Hydroxynitrile lyases (HNLs) catalyze the cleavage as well as the formation of cyanohydrins. The latter reaction is valuable for the stereoselective C-C bond formation by condensation of HCN with carbonyl compounds. The resulting cyanohydrins serve as versatile building blocks for a broad range of chemical and enzymatic follow-up reactions. A significant number of (R)- and (S)-selective HNLs are known today and the number is still increasing. HNLs not only exhibit varying substrate scope but also differ in sequence and structure. Tailor-made enzymes for large-scale manufacturing of cyanohydrins with improved yield and enantiomeric excess are very interesting targets, which is reflected in a solid number of patents. This review will complement and extend our recent review with a strong focus on applications of HNLs for the synthesis of highly functionalized, chiral compounds with newest literature, recent and current patent literature.

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Article Details

VOLUME: 7
ISSUE: 3
Page: [197 - 206]
Pages: 10
DOI: 10.2174/18722083113076660010