Sensitive Detection of Ornidazole by Poly(p-Aminobenzene Sulfonic Acid)- Modified Glassy Carbon Electrode
Ornidazole is an antibacterial drug active compound. A glassy carbon electrode (GCE) was modified with an
electropolymerized film of p-aminobenzene sulfonic acid (p-ABSA) in pH 7.0 phosphate buffer solution (PBS). The electrochemical
properties of the polymer film were studied using cyclic voltammetry (CV) and different pulse voltammetry
(DPV) techniques. The polymer film-modified electrode was used to electrochemically detect ornidazole. The polymer
film showed excellent electrocatalytic activity for the reduction of ornidazole. The cathodic peak potential value of the
ornidazole at the p-ABSA modified glasy carbon electrode was -513 mV obtained by DPV. A linear calibration curve for
DPV analysis was constructed in the ornidazole concentration range 8x10-7-5x10-5 M. Limit of detection (LOD) and limit
of quantification (LOQ) were 1.00x10-7 and 3.32x10-7 M respectively. The DPV technique proposed was successfully applied
to the detection of ornidazole in tablet dosage forms. Precision and accuracy of the developed technique were
checked by recovery studies. The proposed method exhibits good recovery and reproducibility. The mechanisms of electropolymerization
of p-ABSA and reduction of ornidazole at p-ABSA- modified GCE are also suggested.
Keywords: Ornidazole, pharmaceutical formulation, p-Aminobenzene sulfonic acid, modified-glassy carbon electrode, electropolymerization,
electrocatalysis, determination, voltammetry.
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