Letters in Drug Design & Discovery

G. Perry
University of Texas
San Antonio, TX
USA
Email: lddd@benthamscience.org

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Identification of Interfacial Residues Involved in Hepcidin-Ferroportin Interaction

Author(s): Prasoon Kumar Thakur, Amresh Prakash, Parvez Khan, Robert E. Fleming, Abdul Waheed, Faizan Ahmad, Md. Imtaiyaz Hassan.

Abstract:

The interaction of hepcidin (HEPC) and ferroportin (FPN) is an essential event in iron homeostasis. HEPC binds to FPN, and subsequently degrades it through phosphorylation and ubiquitination. Present work described a computational study to identify interfacial residues involved in FPN–HEPC interactions. We have predicted three-dimensional structure of human FPN using homology modeling followed by docking analysis and molecular dynamics simulations. Our study revealed that FPN has HEPC-binding sites on extracellular loops: ECL4, ECL5 and ECL6. The provided outcome is consistent with mutagenesis studies on FPN. We have identified several electrostatic interactions between charged residues Asp325 (ECL4), ECL5 (Glu448) and Glu518 (ECL6) of FPN with residues His3, Arg16, Lys18 of HEPC, which are essentially important for interfacial contact between them. These results provide an evidence for structural basis of FPN–HEPC interactions and their future clinical application.

Keywords: Ferroportin, Hepcidin, Structural modulation, Elastic network model, Iron homeostasis.

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Article Details

VOLUME: 11
ISSUE: 3
Year: 2014
Page: [363 - 374]
Pages: 12
DOI: 10.2174/15701808113106660088
Price: $58