Current Gene Therapy

Ignacio Anegon
Director INSERM UMR 1064-Center for Research in Transplantation and Immunology
CHU de Nantes. 30, boulevard
Nantes
France

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Manganese Superoxide Dismutase Gene Therapy Protects Against Irradiation- Induced Intestinal Injury

Author(s): Chao Yang, Hai-Xu Chen, Yong Zhou, Min-Xia Liu, Yan Wang, Jie-Xi Wang, Su-Ping Ren, Ying Han, Ben-Yan Wu.

Abstract:

Radiation-induced intestinal injury is a common complication in radiotherapy for solid organ malignancies in abdomen or pelvis. However, currently there are no approved medical countermeasures for radiation-induced intestinal injury. Therefore, it is urgent to develop new treatments for radiation-induced intestinal injury. In the present study, we demonstrated that bone marrow derived mesenchymal stem cells (MSCs) and overexpression of human manganese superoxide dismutase (MnSOD) could ameliorate radiation-induced intestinal syndrome. NOD/SCID mice received abdominal irradiation at a selected dose of 5 Gy, and then infused intravenously with MnSOD-MSCs. Mice body weight, survival and diarrhea were monitored for 30-days. Colonization and differentiation of MnSOD-MSCs in the irradiated intestine were analyzed by histological and immunohistochemical methods. Consequently, our data demonstrated that intravenous administration of MnSOD-MSCs improved survival, decreased diarrhea occurrence and protected the small intestinal structural integrity of irradiated mice. Moreover, intravenously transplanted MnSOD-MSCs could colonize the irradiated intestine and repair injured sites. These findings suggested that MnSOD-MSCs may be an attractive and potential option for radiation-induced intestinal injury.

Keywords: Abdominal irradiation, mesenchymal stem cells, manganese superoxide dismutase, small intestinal injury.

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Article Details

VOLUME: 13
ISSUE: 5
Year: 2013
Page: [305 - 314]
Pages: 10
DOI: 10.2174/15665232113136660027
Price: $58