The present study emphasizes the molecular interactions between human brain acetylcholinesterase (AChE)
and the natural ligand Huperzine-B and its comparison to ‘AChE-Tolserine interactions’. Docking between Huperzine-B
and AChE was performed using ‘Autodock4.2’. Hydrophobic interactions and hydrogen bonds both play an equally
important role in the correct positioning of Huperzine-B within the ‘catalytic site’ of AChE to permit docking. However,
docking of Tolserine to AChE is largely dominated by hydrophobic interactions. Such information may aid in the design
of versatile AChE-inhibitors, and is expected to aid in safe clinical use of Huperzine-B. Scope still remains in the
determination of the three-dimensional structure of AChE-Huperzine-B complex by X-ray crystallography to validate the
described data. Furthermore, this study confirms that Huperzine-B is a more efficient inhibitor of human brain AChE
compared to tolserine with reference to Ki and ΔG values.
Keywords: Acetylcholinesterase, autodock4.2, hydrophobic interactions, catalytic site.
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