Molecular Delivery of Plasmids for Genetic Vaccination
Romiza Mazid, Melvin X. Tan and Michael K. Danquah
Affiliation: Department of Chemical Engineering, Curtin University of Technology, Sarawak, Malaysia.
Keywords: Plasmid, Vaccine, Polymer, Immunology, Clinical Trial.
Plasmid vaccination is a smart gene delivery application mostly achieved through the utilisation of viral or copolymeric
systems as surrogated carriers in micro or nano formulations. A common polymeric protocol for plasmid vaccine
formulation, which as somewhat been successful, is via the complexation of the DNA molecules with a cationic
polymer, and encapsulating in a vehicular carrier polymer. Even though plasmid vaccination research has not witnessed
the much anticipated success, due a number of cellular and physicochemical reasons, application of copolymeric carriers
with tight functionalities is a promising strategy to optimally deliver the DNA molecules; in view of the available chemistries
and physical properties that could be tuned to enable enhanced targeted delivery, uptake and specific transfection.
This also enables the targeting of specific epitopes and antigen presenting cells for the treatment of many pathogenic infections
and cancer. This paper provides a brief critical review of the current state of plasmid vaccines formulation and
molecular delivery with analysis of performance data obtained from clinical trials.
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