Interest in stem cell separation and purification from easily accessible clinical specimens is booming due to the
increase of cell therapy applications. The recovery of pluripotent or multipotent stem cells in human sources different
from the embryo requires the use of effective methods of cell sorting/enrichment. Among these sources, perinatal tissues
retain cells with pivotal stem cell features such as high self-renewal ability, wide differentiation potential and immunomodulatory
properties. In this perspective, methods exploiting cell biophysical differences in a less dependent process of
identification based on specific markers are therefore promising. These methods allow cell isolation irrespective of the
broad and diversified surface antigenic panel that usually limits the ability to easily distinguish cells as in the case of mesenchymal
stromal/stem cell separation. In addition, the use of non- or minimally invasive tag-less techniques might be a
way to preserve stem cell features of the selected product and reduce regulatory issues related to their use in regenerative
applications. In this review, non-invasive cell sorting techniques based on microfluidic systems and relevant patents are
described. In particular applications of emerging separation approach, Field-Flow Fractionation (FFF), for perinatal stem
cell sorting are cited. Protocols and applications based on FFF-derived techniques are detailed.
Keywords: Advances in stem cell isolation, field-flow fractionation derived stem cell sorting, microfluidic devices for cell sorting,
non invasive stem cell sorting, patent in tag-less stem cell sorting, tag-less stem cell sorting.
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