Aquilaria agallocha Roxb. family, Thymelaeaceae, is an evergreen plant of South-East Asia, commonly
described as aloe wood or agarwood. Traditionally, the bark, root and heartwood are used for their medicinal properties as
a folk medicine for hundreds of years. Chemical analyses revealed that the bulk of the oil is constituted by agarospirol
(12.5%), jinkoh-eremol (11.8%) and hinesol (8.9%) as major contributor. In the present work, a QSAR model for antiinflammatory
activity of 10-epi-γ-Eudesmol, jinkoh-eremol, agarospirol and other compounds has been developed by
multiple linear regression method. The r2 and rCV2 of a model were 0.89 and 0.81 respectively. In silico molecular
docking study suggests that compound 10-epi--Eudesmol, jinkoh-eremol and agarospirol are preferentially more active
than other identified compounds with strong binding affinity to major anti-inflammatory and immunomodulatory
receptors. The oil displayed a significant and dose dependent reduction of 12-O-tetradecanoylphorobol-13 acetate (TPA)-
induced ear edema and MDA activity when compared with vehicle treated mice. Pro-inflammatory cytokines (IL-1β, IL-6
and TNF-α) were also reduced significantly in a dose dependent manner in all the TPA treated groups as compared to
control. The present study indicates that agarwood oil significantly reduced the skin thickness, ear weight, oxidative stress
and pro-inflammatory cytokines production in TPA-induced mouse ear inflammation model and contributed towards
validation of its traditional use to treat inflammation related ailments.
Keywords: Aquilaria, GC/MS analysis, agarospirol, in silico molecular modelling, ear oedema, docking, QSAR, ADME/T.
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