Protein Microarrays: Technological Aspects, Applications and Intellectual Property
Noelia Dasilva, Paula Díez, María González-González, Sergio Matarraz, J. M. Sayagués, Alberto Orfao and Manuel Fuentes
Affiliation: Centro de Investigación del Cáncer/IBMCC (USAL/CSIC), IBSAL, Departamento de Medicina and Servicio General de Citometría, University of Salamanca, Salamanca 37007, Spain.
Keywords: Antibody, biomarker, capture agent, detection strategy, epitope, fluorochrome, high throughput, ligand, patent, protein
microarray, proteomics, reverse phase array, substrate, surface functionalization, target protein.
Over the last decade, proteomics has undergone remarkable progress thanks to the technical advances made in
the field. Improvements in the design of the protein microarrays, including more types of chemical groups for surface
functionalization, new capture agents and novel detection strategies, among others, have allowed the detection of proteins
in a robust, specific, sensitive, real time and high throughput manner. However, there are still problems that hinder the
analysis of low abundance proteins or those present in complex samples. For this reason, the development of patents related
to the features mentioned above has an important relevance. In this review, we focus on the study of recently approved
patents that try to solve the existing problems. Thanks to them, it is expected that the identification of disease biomarkers
can be made in a suitable and reliable way, and above all, biocompatible and environmentally friendly.
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