Atomic Force Microscopy and Anodic Porous Allumina of Nucleic Acid Programmable Protein Arrays

Author(s): Claudio Nicolini, Tercio Bezerra Correia, Enrico Stura, Claudio Larosa, Rosanna Spera, Eugenia Pechkova.

Journal Name: Recent Patents on Biotechnology

Volume 7 , Issue 2 , 2013

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Abstract:

The methodological aspects are here presented for the NAPPA (Nucleic Acid Programmable Protein Arrays) characterization by atomic force microscopy and anodic porous alumina. Anodic Porous Alumina represents also an advanced on chip laboratory for gene expression contained in an engineered plasmid vector. The results obtained with CdK2, CDKN1A, p53 and Jun test genes expressed on NAPPA and the future developments are discussed in terms of our pertinent and recent Patents and of their possibility to overcome some limitations of present fluorescence detection in probing protein-protein interaction in both basic sciences and clinical studies.

Keywords: Atomic force microscopy, anodic porous alumina, NAPPA.

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Article Details

VOLUME: 7
ISSUE: 2
Year: 2013
Page: [112 - 121]
Pages: 10
DOI: 10.2174/18722083113079990003

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PDF: 11