Mining Sarcomas by Proteomics Approaches: Ewing Sarcoma on the Spotlight
Carlos Mackintosh and Juan Madoz-Gúrpide
Affiliation: Molecular Pathology Program, Centro de Investigación del Cáncer-IBMCC, Universidad de Salamanca- CSIC, Salamanca, 37007, Spain.
Sarcomas are a class of tumors defined by their mesenchymal origin that comprise very different neoplasms.
Although some sarcomas harbor pathogenomic molecular alterations (i.e. specific balanced translocations and their associated
chimeric fusion genes), others still lack an ultimate diagnostic tool, which could be of great interest as in some
cases different sarcomas share a similar clinical manifestation. High throughput tools are contributing new ways to
molecularly delineate the boundaries of each sarcoma subtype. Moreover, they are also shedding light into other research
subjects of immediate concern: (i) the elucidation of the molecular targets of chimeric fusion proteins and their interactome;
(ii) the discovery of new biomarkers and therapeutic targets; and (iii) the delineation of the response to therapeutic
agents. Here we review the application of proteomics approaches to sarcomas, with special emphasis in Ewing sarcoma.
Proteomics strategies offer the focus, the analytical potential, and the high throughput capabilities to decipher the hidden
agenda of the biology of sarcomas, a knowledge that will surely be the subject of future patents intended to develop new
diagnostic and therapeutic tools.
Keywords: 2-DE, Cancer, cell signaling, chimeric fusion protein, chromosomal translocation, diagnostic tools, DIGE, Ewing
sarcoma, EWS-FLI1, MALDI-ToF, mass spectrometry, molecular targets, oncogene, protein microarrays, proteomics, sarcomas,
sarcomagenesis, targets of therapy.
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