MicroRNAs (miRNAs) participate in the regulation of myogenesis and muscle physiological function.
Most skeletal muscles in vertebrates contain a mixture of fibertypes. So far, the regulatory mechanism of the
miRNA in terms of controlling muscle phenotype is poorly understood. In the present study, we use Siniperca
chuatsi as a model system and demonstrate that miRNAs are involved in regulating the physiological
processes and metabolism of different muscle fibers in vertebrates. The miRNA transcriptomes of the white
muscle, red muscle, and five other tissues from Siniperca chuatsi were profiled using Solexa deep sequencing.
We characterized 186 conserved miRNAs and 3 novel miRNAs from the two small RNA libraries of white and
red muscles. Among the 155 miRNAs overlapped between the two libraries, we identified 60 significantly
expressed miRNAs between the two types of muscle fibers. Using integrative miRNA target-prediction and
network-analysis approaches, an interaction network of differentially expressed and muscle-related miRNAs
and their putative targets were constructed. Sch-miR-181a-5p that could act to control the performance of the
different muscle fiber types by targeting the myostatin gene was identified.
Keywords: Differential expression, microRNA, muscle fiber, myostatin, regulation, Siniperca chuatsi.
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