Protein Serine/Threonine Phosphotase-2A is Differentially Expressed and Regulates Eye Development in Vertebrates

Author(s): W.-B. Liu, X.-H. Hu, X.-W. Zhang, M.-X. Deng, L. Nie, S.-S. Hui, W. Duan, M. Tao, C. Zhang, J. Liu, W.-F. Hu, Z.-X. Huang, L. Li, M. Yi, T.-T. Li, L. Wang, Y. Liu, S.-J. Liu, D. W.-C. Li.

Journal Name: Current Molecular Medicine

Volume 13 , Issue 8 , 2013

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Abstract:

Protein serine/threonine phosphatase-2A (PP-2A) is one of the key enzymes responsible for dephosphorylation in vertebrates. PP-2A-mediated dephosphorylation participates in many different biological processes including cell proliferation, differentiation, transformation, apoptosis, autophage and senescence. However, whether PP-2A directly controls animal development remains to be explored. Here, we present direct evidence to show that PP-2A displays important functions in regulating eye development of vertebrates. Using goldfish as a model system, we have demonstrated the following novel information. First, inhibition of PP-2A activity leads to significant death of the treated embryos, which is derived from blastomere apoptosis associated with enhanced phosphorylation of Bcl-XL at Ser-62, and the survived embryos displayed severe phenotype in the eye. Second, knockdown of PP-2A with morpholino oligomers leads to significant death of the injected embryos. The survived embryos from PP-2A knockdown displayed clear retardation in lens differentiation. Finally, overexpression of each catalytic subunit of PP-2A also causes death of majority of the injected embryos and leads to absence of goldfish eye lens or severely disturbed differentiation. Together, our results provide direct evidence that protein phosphatase-2A is important for normal eye development in goldfish.

Keywords: Apoptosis, differentiation, eye development, lens, PP-2A, PP-2Acα/β, protein phosphatases, retina.

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Article Details

VOLUME: 13
ISSUE: 8
Year: 2013
Page: [1376 - 1384]
Pages: 9
DOI: 10.2174/15665240113139990061
Price: $58

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