Identification of a Novel Antimicrobial Peptide from Brazilian Coast Coral Phyllogorgia dilatata
Loiane Alves de Lima,
Clovis Barreiro e Castro,
Debora de Oliveira Pires,
Eveline Ferreira Goncalves,
Ilka Maria Vasconcelos,
Jose Tadeu Abreu de Oliveira,
Anselmo de Jesus Otero-Gonzalez,
Octavio Luiz Franco,
Simoni Campos Dias.
The marine ecosystem is able to provide enormous biomolecule diversity that could be used for treatment of
various diseases. In this highly competitive environment, organisms need chemical barriers to reduce or avoid microorganism
contamination. Among the molecules that protect these animals the antimicrobial peptides (AMPs) are included.
In the present study, crude extracts of coral coral specimens Carijoa riisei, Muriceopsis sulphurea, Neospongodes atlantica,
Palythoa caribeorum, Phyllogorgia dilatata and Plexaurella grandiflora were challenged against multiple Grampositive
and -negative bacteria showing different activities. P. dilatata crude extract showed the antibacterial activity, and
was ammonium-sulfate (0-40%) fractionated, being able to control the growth of K. pneumoniae, S. flexineri and S.
aureus. Rich-fraction was further purified by using Amicon® Ultra Centrifugal 10 kDa associated with reversed-phase
HPLC chromatography (C18), producing the peptide named Pd-AMP1. Pd-AMP1 was able to inhibit S. aureus development.
Mass spectrometry analyses showed a monoisotopic mass of 5372.66 Da and N-terminal sequence showed no
significant match with databank. In this view, the prospecting of protein biomolecules and biotechnological potential from
marine animals is still little explored and may serve as an alternative to common antibiotics.
Keywords: Reef coral, Phyllogorgia dilatata, peptides, human pathogenic bacteria.
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