Identification of a Novel Antimicrobial Peptide from Brazilian Coast Coral Phyllogorgia dilatata

Author(s): Loiane Alves de Lima, Ludovico Migliolo, Clovis Barreiro e Castro, Debora de Oliveira Pires, Carlos Lopez-Abarrategui, Eveline Ferreira Goncalves, Ilka Maria Vasconcelos, Jose Tadeu Abreu de Oliveira, Anselmo de Jesus Otero-Gonzalez, Octavio Luiz Franco, Simoni Campos Dias.

Journal Name:Protein & Peptide Letters

Volume 20 , Issue 10 , 2013

Abstract:

The marine ecosystem is able to provide enormous biomolecule diversity that could be used for treatment of various diseases. In this highly competitive environment, organisms need chemical barriers to reduce or avoid microorganism contamination. Among the molecules that protect these animals the antimicrobial peptides (AMPs) are included. In the present study, crude extracts of coral coral specimens Carijoa riisei, Muriceopsis sulphurea, Neospongodes atlantica, Palythoa caribeorum, Phyllogorgia dilatata and Plexaurella grandiflora were challenged against multiple Grampositive and -negative bacteria showing different activities. P. dilatata crude extract showed the antibacterial activity, and was ammonium-sulfate (0-40%) fractionated, being able to control the growth of K. pneumoniae, S. flexineri and S. aureus. Rich-fraction was further purified by using Amicon® Ultra Centrifugal 10 kDa associated with reversed-phase HPLC chromatography (C18), producing the peptide named Pd-AMP1. Pd-AMP1 was able to inhibit S. aureus development. Mass spectrometry analyses showed a monoisotopic mass of 5372.66 Da and N-terminal sequence showed no significant match with databank. In this view, the prospecting of protein biomolecules and biotechnological potential from marine animals is still little explored and may serve as an alternative to common antibiotics.

Keywords: Reef coral, Phyllogorgia dilatata, peptides, human pathogenic bacteria.

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Article Details

VOLUME: 20
ISSUE: 10
Year: 2013
Page: [1153 - 1158]
Pages: 6
DOI: 10.2174/0929866511320100010
Price: $58