Fluorescent Probes for Cellular Assays
Pp. 60-83 (24)
George T. Hanson and Bonnie J. Hanson
A fluorescent probe is a fluorophore designed to localize within a specific
region of a biological specimen or to respond to a specific stimulus. Fluorescent probes
have been used for nearly a century to study cellular processes due to their exquisite
sensitivity and selectivity. Fluorescent probes have also gained in popularity as safety
and environmental concerns over the use of radioactive probes have grown. At the same
time, cellular assays are being more widely used now than ever before. This review will
give a broad overview of types of fluorescent probes, types of fluorescent assays, and
their application in cellular assays for a number of pharmaceutically relevant target
Fluorescence, cell based assays, high throughput screening, GPCR,
pathway, ion channel, kinase, multiplexing, caspase.
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