N-Glycosylation of Plant-produced Recombinant Proteins

Author(s): Dirk Bosch, Alexandra Castilho, Andreas Loos, Arjen Schots, Herta Steinkellner.

Journal Name: Current Pharmaceutical Design

Volume 19 , Issue 31 , 2013

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Abstract:

Plants are gaining increasingly acceptance as a production platform for recombinant proteins. One reason for this is their ability to carry out posttranslational protein modifications in a similar if not identical way as mammalian cells. The capability of plants to carry out human-like complex glycosylation is well known. Moreover, the targeted manipulation of the plant N-glycosylation pathway allows the production of proteins carrying largely homogeneous, human-type oligosaccharides. These outstanding results have placed plants in a favourable position compared to other eukaryotic expression systems. This review provides a comprehensive summary of the N-glycosylation of plant-produced recombinant proteins, the possible impact of plant-specific N-glycans on the human immune system, and recent advances in engineering the plant N-glycosylation pathway towards the synthesis of (complex) human-type glycan structures, highlighting challenges and achievements in the application of these powerful technologies.

Keywords: Plants, recombinant proteins, glycosylation, glyco-engineering.

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Article Details

VOLUME: 19
ISSUE: 31
Year: 2013
Page: [5503 - 5512]
Pages: 10
DOI: 10.2174/1381612811319310006

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