Effects of Senna didymobotrya Root Extract and Compounds on Tritoninduced Hyperlipidaemic Rats and Differentiation of 3T3-Li Preadipocytes
Charles O. Ochieng,
Ashok K. Khanna,
Rakesh K. Asthana,
Philip O. Owuor,
Lawrence A. Manguro,
Anil K. Saxena.
The lipid lowering effects of Senna didymobotrya (Leguminoceae) root extract (250 mg/kg) and pure isolates
(100 mg/kg) were investigated for hypolipidemic activity on Triton-induced hyperlipidemic rats (in-vivo). The extracts
and isolates showed significant (p≤0.01) reduction in serum lipid contents, and reactivation of the lipolytic enzymes,
relative to the Triton-treated group. Ethyl acetate extract exhibited the highest lipid lowering (p≤0.01) compared to other
fractions. Seven anthraquinones, obtusifolin (1), 1,6-di-O-methylemodin (2), nataloemodin-8-methyl ether (3),
chrysophanol (4), physcion (5), chrysophanol-10,10'-bianthrone (6), physcion-10,10-bianthrone (7) and stigmasterol (8)
were isolated from the ethyl acetate extract. Compounds 3, 5 and 7 displayed significant (p≤0.01) lipid lowering effects.
The hypolipidaemic activities prompted the determination of inhibition of differentiation of 3T3-L1 preadipocytes into
adipocytes by the compounds 3, 5 and 7. Only compound 7 exhibited potent anti-adipogenic activity without significant
cytotoxicity. The result indicated that extracts of S. didymobotrya root extract can provide good antidyslipidaemic and
adipocytes maturation inhibitors.
Keywords: Adipogenesis, antioxidants, hyperlipidaemia, leguminocea, senna didymobotrya.
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