Abstract
Context: In folk medicine the dried fruits of Elaeocarpus ganitrus Roxb. (Elaeocarpaceae), have been used in the treatment of melancholia, manic conditions and other psychological disorders.
Objective: To isolate the anxiolytic constituent of E. ganitrus using bioactivity guided fractionation.
Materials and methods: Crude extracts of E. ganitrus fruits were prepared using petroleum ether, chloroform and ethanol in a Soxhlet apparatus and finally the marc was boiled with water. Dried extracts were evaluated (50, 100, 200, 400 or 800 mg/kg, p.o) for antianxiety activity in mice using elevated plus maze apparatus (EPM). The most active ethanol extract (1000, 2000, 3000, 5000 mg/kg) was given to animals to observe acute toxicity, and observed for 14 days. Further the bioactive ethanol extract was subjected to column chromatography to isolate the bioactive constituent. The isolated constituent was further evaluated for antianxiety activity using EPM assay, light/dark chamber and double mirrored chamber model of anxiety. The structure of isolated compound was elucidated by IR, 1H NMR, and 13C NMR spectral data.
Results: Results showed that no mortality was reported even after 14 days. This indicated that ethanol extract was safe up to a single dose of 5000 mg/kg p.o. Antianxiety activity guided fractionation of ethanol extract led to the isolation of 3,3',4',5,7-pentahydroxyflavone. The above isolated bioactive compound (25 mg/kg, p.o) exhibited significant anxiolytic activity in mice using EPM assay, light/dark chamber and double mirrored chamber model of anxiety.
Conclusion: It is concluded that 3,3',4',5,7-pentahydroxyflavone is responsible for antianxiety activity of traditionally used E. ganitrus.
Keywords: Antianxiety, central nervous system, elaeocarpus, flavonoids, rudraksa, quercetin.
The Natural Products Journal
Title:Bioactivity Guided Isolation of Quercetin as Anxiolytic Compound from Elaeocarpus ganitrus Beads
Volume: 3 Issue: 3
Author(s): Balbir Singh, Vishal Sharma, Mohan Pal Singh Ishar and Anupam Sharma
Affiliation:
Keywords: Antianxiety, central nervous system, elaeocarpus, flavonoids, rudraksa, quercetin.
Abstract: Context: In folk medicine the dried fruits of Elaeocarpus ganitrus Roxb. (Elaeocarpaceae), have been used in the treatment of melancholia, manic conditions and other psychological disorders.
Objective: To isolate the anxiolytic constituent of E. ganitrus using bioactivity guided fractionation.
Materials and methods: Crude extracts of E. ganitrus fruits were prepared using petroleum ether, chloroform and ethanol in a Soxhlet apparatus and finally the marc was boiled with water. Dried extracts were evaluated (50, 100, 200, 400 or 800 mg/kg, p.o) for antianxiety activity in mice using elevated plus maze apparatus (EPM). The most active ethanol extract (1000, 2000, 3000, 5000 mg/kg) was given to animals to observe acute toxicity, and observed for 14 days. Further the bioactive ethanol extract was subjected to column chromatography to isolate the bioactive constituent. The isolated constituent was further evaluated for antianxiety activity using EPM assay, light/dark chamber and double mirrored chamber model of anxiety. The structure of isolated compound was elucidated by IR, 1H NMR, and 13C NMR spectral data.
Results: Results showed that no mortality was reported even after 14 days. This indicated that ethanol extract was safe up to a single dose of 5000 mg/kg p.o. Antianxiety activity guided fractionation of ethanol extract led to the isolation of 3,3',4',5,7-pentahydroxyflavone. The above isolated bioactive compound (25 mg/kg, p.o) exhibited significant anxiolytic activity in mice using EPM assay, light/dark chamber and double mirrored chamber model of anxiety.
Conclusion: It is concluded that 3,3',4',5,7-pentahydroxyflavone is responsible for antianxiety activity of traditionally used E. ganitrus.
Export Options
About this article
Cite this article as:
Singh Balbir, Sharma Vishal, Singh Ishar Pal Mohan and Sharma Anupam, Bioactivity Guided Isolation of Quercetin as Anxiolytic Compound from Elaeocarpus ganitrus Beads, The Natural Products Journal 2013; 3 (3) . https://dx.doi.org/10.2174/22103155113039990010
DOI https://dx.doi.org/10.2174/22103155113039990010 |
Print ISSN 2210-3155 |
Publisher Name Bentham Science Publisher |
Online ISSN 2210-3163 |
Call for Papers in Thematic Issues
Ayurvedic medicinal therapeutic approaches for inflammatory and neurodegenerative disorders
Usually, in our elderly population, neurodegenerative disorders occur due to neuron death. Alzheimer?s disease (AD) is an irreversible and progressive neurodegenerative disease. Dementia is the leading cause of AD, which affects about 30 million people worldwide. According to the World Alzheimer report 2018, a new case of dementia develops every ...read more
Recent Advances in Biotransformation of Bioactive Terpenoids using Aspergillus niger
Biotransformation is the reaction catalyzed by biological catalysts of either isolated enzymes or whole cell cultures of microorganisms. This process has been extensively applied in the conversion of bioactive terpenoids with high regio- and stereospecifity. This process has been also applied in the lead discovery and development. Aspergillus niger has ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Contribution of Inflammatory Processes to Nerve Cell Toxicity by Bilirubin and Efficacy of Potential Therapeutic Agents
Current Pharmaceutical Design Inherited Copper Transport Disorders: Biochemical Mechanisms, Diagnosis, and Treatment
Current Drug Metabolism Organic Lesions in the Brain MRI of Children with Febrile Seizure
Current Medical Imaging Recent Trends in the Discovery of Small Molecule Blockers of Sodium Channels
Current Medicinal Chemistry Inhibitors of Aldo-Keto Reductases AKR1C1-AKR1C4
Current Medicinal Chemistry Histamine H3 Antagonists for Treatment of Cognitive Deficits in CNS Diseases
Current Topics in Medicinal Chemistry Role of Unani Medicines in Cancer Control and Management
Current Drug Therapy Therapeutic Potential, Synthesis, Patent Evaluation and SAR Studies of Thieno[3,2-d]pyrimidine Derivatives: Recent Updates
Current Drug Targets Organotypic Cultures as Tools for Testing Neuroactive Drugs – Link Between In-Vitro and In-Vivo Experiments
Current Medicinal Chemistry The Treatment of Painful Diabetic Neuropathy
Current Diabetes Reviews Nonconvulsive (Dialeptic) Status Epilepticus in Children
Current Pediatric Reviews Modulation of Potassium Channels as a Therapeutic Approach
Current Pharmaceutical Design The Evolution of Histamine H3 Antagonists/Inverse Agonists
Current Topics in Medicinal Chemistry Neurotoxicity and Side-Effects of Highly Active Antiretroviral Therapy [HAART] on the Central and Peripheral Nerve System
Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry Epidemiology of Major Congenital Malformations with Specific Focus on Teratogens
Current Drug Safety Poly(ADP-Ribose)Polymerase 1 (PARP-1) Activation and Ca<sup>2+</sup> Permeable α-Amino-3-Hydroxy-5-Methyl-4-Isoxazolepropionic Acid (AMPA) Channels in Post-Ischemic Brain Damage: New Therapeutic Opportunities?
CNS & Neurological Disorders - Drug Targets Treatment of Central Nervous System Tuberculosis Infections and Neurological Complications of Tuberculosis Treatment
Current Pharmaceutical Design Protein Kinase C Pharmacology: Perspectives on Therapeutic Potentials as Antidementic and Cognitive Agents
Recent Patents on CNS Drug Discovery (Discontinued) Parkinson Disease Genetics: A "Continuum" from Mendelian to Multifactorial Inheritance
Current Molecular Medicine Patient-Specific Induced Pluripotent Stem Cell Models in Mitochondrial Diseases
Current Stem Cell Research & Therapy