Protein Arrays: Recent Achievements and their Application to Study the Human Proteome
Juan Casado-Vela, Maria Gonzalez-Gonzalez, Sergio Matarraz, Maria Jose Martinez-Esteso, Maite Vilella, Jose Maria Sayagues, Manuel Fuentes and Juan Carlos Lacal
Affiliation: Centro Nacional de Biotecnología (CNB), Spanish National Research Council (CSIC). Darwin, 3. 28049 Madrid.
Protein arrays represent a step forward in proteomic technologies, as they enable to address biological and
functional questions by large-scale approaches (i.e., up to several thousand proteins may be monitored in a single experiment).
Here we review recent achievements of protein arrays and their classification. We also summarize current and future
applications of this technology to address the study of the human proteome including protein identification, biomarker
discovery, screening of protein-protein/molecule interactions, detection of protein post-translational modifications
and serum profiling. Advantages and limitations of protein arrays compared to other proteomic technologies are also discussed
here. The use, advances and recent applications of fluorescent microspheres as a promising alternative to planar arrays
are also summarized in this report. Finally, we propose future perspectives of protein arrays from a comprehensive
standpoint that includes technological, biological, economical and practical aspects.
Keywords: Advantages and limitations of protein arrays, antibody characterization, array classification, cell-free protein synthesis,
human proteome, microspheres, nanoparticles, NAPPA, perspectives, protein arrays, proteomics.
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