Advances of an Infrared Hyperspectral Spectroscopy Platform for Medicinal Plant Materials Analysis and its Impact on In vivo Studies
C. Pezzei, B. Kopp, H. Stuppner and C. W. Huck
Pages 235-246 (12)
Fourier transform infrared (FTIR) spectroscopy and especially FTIR spectroscopic imaging are suitable in
the plant analysis. With these techniques, a distinction not only of different plant species, but also of various ingredients
within a plant is possible. FTIR spectroscopic imaging enables molecular imaging of complex botanical samples
and therefore the detection and characterization of the molecular components of biological tissue. Based on the absorption
of IR radiation by vibrational transitions in covalent bonds, the FTIR spectroscopic imaging technique offers the
major advantage of the acquisition of local molecular expression profiles, while maintaining the topographic integrity
of the tissue by avoiding time-consuming extraction, purification and separation steps. In the present study, it is demonstrated
that FTIR spectroscopy enables the distinction of various plant species and FTIR spectroscopic imaging
combined with chemometric data analysis enables one to gain a more distinct picture concerning morphology and distribution
of active ingredients. In addition, initial evaluations of a three-dimensional distribution of ingredients of
various plant roots are shown.
Fourier transform infrared spectroscopy, near infrared spectroscopy, imaging, mapping.
Head of Spectroscopy Group, Institute of Analytical Chemistry and Radiochemistry, CCB - Center for Chemistry and Biomedicine, Leopold-Franzens University, Innrain 80/82, 6020 Innsbruck, Austria.