Biosynthesis of Selenium Nanoparticle by Whole Cells of Saccharomyces boulardii and its Evaluation as Anticancer Agent
Uttam Chand Banerjee.
Selenium is known to have cytoprotective role, which is important especially in reducing mortality due to cancer, a leading
cause of death worldwide. The present work addresses synthesis and evaluation of selenium nanoparticles (SeNPs) as therapeutic alternative
to conventional chemotherapeutic agents. Selenium nanoparticles were synthesized by an eco friendly method using whole cells of
Saccharomyces boulardii. Parameters like culture age (stationary phase growth), cell mass concentration (100 mg/ml), temperature
(35°C) and reaction time (72 h) were optimized to produce nanoparticle with controlled properties. Nanoparticles were recovered by alkali
hydrolysis and subjected to UV-Vis spectroscopy, EDX (energy dispersive X-rays) analysis, electron microscopy, elemental analysis
and size determination. Dynamic light scattering analysis reveals negative zeta potential value of 14.8 mV and PDI of 0.25. Experiments
to prove possible involvement of sulfite reductase in nanoparticle formation has been carried out. Moreover, cytoprotective role of selenium
nanoparticle has been shown by cell viability in MTT assay.
Keywords: Cytoprotective, MTT assay, Saccharomyces boulardii, Selenium nanoparticle, Sulfite reductase.
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