Green Synthesis of Silver Nanoparticles Using Citrus Reticulata Juice and Evaluation of their Antibacterial Activity and Cytotoxicity Against Melanoma-B16/F10 Cells
P. C. Nagajyothi,
Kap Duk Lee.
The present study deals with the green synthesis of silver nanoparticles (AgNPs) using Citrus reticulata juice as the reducing
agent. The reaction carried out at room temperature (25°C) and at 55°C. The AgNPs were characterized by UV-Vis, TEM, XRD, AFM
and FTIR measurements. The produced AgNPs were also evaluated for their increased antibacterial activities with tetracycline and penicillin
against Gram-positive and Gram-negative bacteria. In this study, combination of AgNPs (at 55°C) and penicillin showed high inhibition
zone in E. coli (18 mm) compared to that of AgNPs (at 25°C) and tetracycline. The cytotoxic effects of AgNPs from the Citrus reticulate
juice on B16/ F10 melanoma cell line were evaluated by MTT (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide)
assay. MTT assay showed a minimum cell inhibition (14.83%) at 0.125 mM (AgNPs at 25°C) and a maximum cell inhibition (31.29%)
was observed at 1 mM (AgNPs at 55°C).
Keywords: Green synthesis, Citrus reticulata juice, AgNPs, antibacterial studies, cytotoxicity.
Rights & PermissionsPrintExport