Electrochemical Study of Ellipticine Interaction with Single and Double Stranded Oligonucleotides
Ellipticine (5,11-dimethyl-6H-pyrido[4,3-b]carbazole) is an alkaloid that has been isolated from plants of an Apocynaceae
family. It is one of the simplest naturally occurring alkaloids with a planar structure. Over the past decades, ellipticine became a very
promising antitumor agent. Interaction with DNA is one of the most studied ellipticine effects on cell division. This phenomenon is not
clearly explained so far. In our experiments we studied interaction of ellipticine with single-stranded and double-stranded oligonucleotides by
electrochemical methods on mercury electrode. Differential pulse voltammetry was applied for ellipticine (Elli) and CA peak detection.
Square wave voltammetry was applied for G peak detection. The effect of the interaction time and ellipticine concentrations on
interactions of ellipticine with single- and double-stranded oligonucleotides was tested too.
Keywords: Adsorptive transfer stripping technique, DNA, differential pulse voltammetry, ellipticine, hetero-nucleotides, intercalation, square
wave voltammetry, cancer, therapy.
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