Recent revolutionary developments in luciferase engineering technologies now allow researchers to carry out
quantitative imaging of molecular dynamics and cell signaling in living subjects, the technology of which includes
protein-fragment complementation assay (PCA) and intein-mediated protein splicing assay (PSA). These technologies are
functionally optimized with specific marine and beetle luciferases exerting designed properties and functionalities. This
review overviews how to fabricate such bioluminescent tools and technologies of reconstituting split-luciferases, providing an
excellent platform for today’s molecular imaging.
Keywords: Bioluminescence, luciferase, protein-fragment complementation assay (PCA), single-chain probes.
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