Generic placeholder image

Current Molecular Imaging (Discontinued)

Editor-in-Chief

ISSN (Print): 2211-5552
ISSN (Online): 2211-5544

Fabrication of Split-Luciferase Complementation Assays for Molecular Imaging

Author(s): Sung Bae Kim and Takeaki Ozawa

Volume 2, Issue 2, 2013

Page: [148 - 157] Pages: 10

DOI: 10.2174/22115552113029990001

Price: $65

Abstract

Recent revolutionary developments in luciferase engineering technologies now allow researchers to carry out quantitative imaging of molecular dynamics and cell signaling in living subjects, the technology of which includes protein-fragment complementation assay (PCA) and intein-mediated protein splicing assay (PSA). These technologies are functionally optimized with specific marine and beetle luciferases exerting designed properties and functionalities. This review overviews how to fabricate such bioluminescent tools and technologies of reconstituting split-luciferases, providing an excellent platform for today’s molecular imaging.

Keywords: Bioluminescence, luciferase, protein-fragment complementation assay (PCA), single-chain probes.


Rights & Permissions Print Cite
© 2024 Bentham Science Publishers | Privacy Policy