Pharmacokinetic Profile of A New Diclofenac Prodrug without Gastroulcerogenic Effect

Author(s): Michel Leandro de Campos , Helen Mariana Baldan-Cimatti , Marcelo Gomes Davanco , Marco Antonio Ferraz Nogueira , Elias Carvalho Padilha , Caroline Damico Candido , Jean Leandro dos Santos , Rosangela Goncalves Peccinini .

Journal Name: Drug Metabolism Letters

Volume 6 , Issue 4 , 2012

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Gastrotoxicity is a major problem for long-term therapy with non-steroidal anti-inflammatory drugs (NSAIDs). DICCIC (1-(2,6-dichlorophenyl)indolin-2-one) is a new diclofenac prodrug, which has proven anti-inflammatory activity without gastroulcerogenic effect. The aim of this work was to compare the pharmacokinetic profiles of diclofenac from DICCIC (7.6 mg/kg equivalent to 8.1 mg/kg diclofenac) and diclofenac (8.1 mg/kg) administration in Wistar rats weighing 250-300 g (n=20). The doses were calculated by interspecific allometric scaling based on the 2 mg/kg from diary human dose of diclofenac. Blood samples were collected in heparinized tubes via the femoral artery through the implanted catheter. The plasma was separated and quantitation was made in a HPLC system with a UV-Vis detector. The confidence limits of the bioanalytical method were appropriate for its application in a preclinical pharmacokinetic study. The AUC of diclofenac from DICCIC (53.7± 5.8 ug/mL.min) was significantly less (Mann Whitney test, p<0.05) than that of diclofenac from diclofenac administration (885.9 ± 124,8 ug/mL.min). Terminal half-life of diclofenac from DICCIC (50.1 ± 17.2 min) was significantly less (Mann Whitney test, p<0.05) than that of diclofenac from diclofenac administration (247.4 ± 100.9 min). Still the parameters clearance and distribution volume were calculated for diclofenac from diclofenac, whose results were 9.2 ±1.2 mL/ and 3.3 ±1.2 L/kg, respectively. The results of DICCIC from DICCIC administration were 108.9 ± 19.6 mL/ and 7.8 ± 2.4 L/kg for clearance and distribution volume, respectively. The pharmacokinetic profile demonstrated that there was an increase in diclofenac elimination and a lower exposure to diclofenac with administration of DICCIC compared to diclofenac.

Keywords: Diclofenac prodrug, 1-(2, 6-dichlorophenyl)indolin-2-one, preclinical pharmacokinetic profile, bioanalytical method.

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Article Details

Year: 2012
Page: [235 - 241]
Pages: 7
DOI: 10.2174/1872312811206040002
Price: $58

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