Role of HBV Replication in Host Cell Metabolism: A Proteomics Analysis
Tiong Keng Low,
Wei Ning Chen.
Hepatocellular carcinoma (HCC) with more than 700,000 deaths every year is the most prevalent type of liver
cancer and a global concern. It is the fifth most common cancer worldwide and has a poor general prognosis. Chronic
hepatitis B virus (HBV) infection is a major cause of HCC. The HBV-infected individual has 100 times higher risk of developing
HCC. The x protein of HBV (HBx) has been shown to involve in the development of HCC. In this study, the association
between HBV replication and the host cell metabolism is investigated. HepG2 cells are transfected with different
genotypes of HBx and total proteins are extracted and analyzed using LC-MS/MS. Our proteomics results indicates that a
number of glycolytic enzymes including glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate kinase (PK),
Phosphoglyceratekinase (PGK) and Lactate dehydrogenase (LDH) are significantly up-regulated in HepG2 cells transfected
by HBx comparing with control group. These findings suggest that HBV replication could alter host cell metabolism
by increasing the rate of glycolysis to provide important metabolic requirements for nucleotides, amino acids and lipids
synthesis. Hence, our proteomics approach may provide candidate biomarkers to improve the diagnosis of HBVrelated
Keywords: Biomarker, HBV, HBx, HCC, LC-MS/MS, metabolic enzymes, proteomics.
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