Wharton's Jelly Mesenchymal Stem Cell Response on Chitosan-graft-poly (ε-caprolactone) Copolymer for Myocardium Tissue Engineering
Cell therapy and tissue engineering attract increasing attention as a potential approach for cardiac repair. Although a plethora
of interesting concepts in the emerging field of cardiac stem cell-based tissue engineering are reported, there are still challenges that this
field needs to overcome to achieve therapeutic translation into the clinical praxis. Engineering biomaterial scaffolds that facilitate stem
cell engraftment, survival and homing are crucial for successful cellular cardiomyoplasty after myocardial infarction (MI). In this study
we investigate for the first time the cellular response of Wharton’s jelly (WJ) Mesenchymal Stem Cells (MSCs) on a copolymeric material
comprising chitosan (CS) and poly(ε-caprolactone) (PCL). First we synthesize a copolymer consisting of poly(ε-caprolactone) grafted
on a chemically modified chitosan-backbone (CS-g-PCL). Furthermore, we investigate the morphology, viability and proliferation of WJMSCs
on material coatings and examine the cellular response from different donors. Our results show strong cell adhesion on the CS-g-
PCL material surface from the first hours in culture, and a proliferation increase after 3 and 7 days. These findings support the potential
use of our proposed cell-material combination in myocardium tissue engineering.
Keywords: Wharton's jelly, chitosan grafted poly(ε-caprolactone), cell adhesion, viability, proliferation, myocardium regeneration.
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