Azo dyes, which are considered as the most recalcitrant and persistent among all groups of dye, were able to be
biodegraded by various types of bacteria. The biodegradation efficiency can be improved by applying immobilization
technology on specific bacteria. In this study, a novel approach by developing a thin lm carried with bacteria and attached
on a carrier was used to study the azo dye degradation. The paint-PVA biofilm was formed and provided with an
alternative and a potentially practical use. However, the cell activities required further recovery operations prior to use in
biodegradation of azo dye. The optimal temperature of paint-PVA immobilized cells showed the best performance on biodegradation
at 35°C to 37°C, the identical optimal temperature range with Ca-alginate immobilized cells, but the favorable
range of pH is quite large which is from 5 to 9. In this system, the kinetics was also investigated to obtain the values of
Vmax of 2.66 mg g-1 h-1 and Km of 161 mg L-1. The slower biodegradation rate was explained by small value of diffusion
coefficient of 2.12 x 10-7 cm s-1. The immobilized biofilm showed a high stability in re-use for dye degradation.
Keywords: Azo dye, biodegradation, immobilization, paint-PVA.
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