Investigation of 5-HT3A Receptor Gene Expression in Peripheral Blood Mononuclear Cells of Individuals who had been Exposed to Air Pollution

Author(s): Ghasem Ahangari, Leila Mohammadi Amirabad, Sona Mozafari, Ali Majeidi, Gholamreza Derkhshan Deilami.

Journal Name: Inflammation & Allergy - Drug Targets (Discontinued)

Volume 12 , Issue 6 , 2013

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Abstract:

The role of air pollution in exacerbation of allergic symptoms is well known. Several studies have shown the effect of air pollution on serotonergic system. The changes in serotonergic system could trigger several allergic symptoms. 5-HT3A is among serotonin receptors on the peripheral Blood Mononuclear Cells (PBMCs) as well as other cells.

In the present study we compared the 5-HT3A gene expression in PBMCs of the asthmatic patients as well as individuals who had been exposed to the air pollution. Normal individuals were also included in the study as control for comparison of 5-HT3A gene expression. Following the synthesis of the cDNA using mRNA extracted from PBMCs the level of 5- HT3A gene expression was measured using real-time PCR. The results showed t a significant increase in the relative expression level of 5-HT3A receptor in PBMCs from asthmatic patients and individuals exposed to the air pollutants compared to normal controls. Our result indicates that significant increase in 5-HT3A receptor may contribute to the pathogenesis as well as allergic symptoms which resulted from air pollution.

Keywords: 5-HT3A receptor, air pollution, allergy, asthma, expression, Real-time PCR.

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Article Details

VOLUME: 12
ISSUE: 6
Year: 2013
Page: [433 - 438]
Pages: 6
DOI: 10.2174/18715281113129990041
Price: $58

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