The pH-Triggered Conversion of the PrPc to PrPsc

Author(s): Guo-Ping Zhou, Ri-Bo Huang.

Journal Name: Current Topics in Medicinal Chemistry

Volume 13 , Issue 10 , 2013

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Abstract:

Transmissible spongiform encephalopathies (TSEs) are prion protein misfolding diseases that involve the accumulation of an abnormal β-sheet-rich prion protein aggregated form (PrPsc) of the normal α- helix-rich prion protein (PrPc) within the central nervous system (CNS) and other organs. On account of its large size and insolubility properties, characterization of PrPc is quite difficult. A soluble intermediate, called PrPβ or βo, exhibiting many of the same features as PrPsc, can be generated using a combination of low pH and/or mild denaturing conditions. Here, we review the current knowledge on the following five issues relevant to the conversion mechanisms of PrPc to PrPsc : (1) How is the Stability of the Helical Structures in the Native PrPc Related to the Primary Structure of the PrPc (2) Why the Low pH Solution System is a Ideal Trigger of PrPc to PrPsc Conversion (3) How are the Structural and Dynamical Characteristics of the α-helixrich Intermediates Determined using NMR Data (4) How are the Premolten (PrPα4 and PrPαβ) and β-Oligomer (PrPβ) Intermediates Detected and Assayed, and (5) Can the Disordered N-terminal Domain be folded into the Structural Segment? Particularly, Chou’s wenxiang diagram (http://en.wikipedia.org/wiki/Wenxiang_diagram) was introduced for providing an intuitive picture. This review may help to further understand the prion protein misfolding mechanism.

Keywords: PrP, protein misfolding, NMR spectroscopy, CD, β-oligomer, premolten globule state, salt-bridge network, Chou’s wenxiang diagram.

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Article Details

VOLUME: 13
ISSUE: 10
Year: 2013
Page: [1152 - 1163]
Pages: 12
DOI: 10.2174/15680266113139990003
Price: $58

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