Purification and kinetics study of thermostable cellulase free Xylanase from Bacillus subtilis

Author(s): Muhammad Irfan, Muhammad Nadeem, Quratualain Syed.

Journal Name: Protein & Peptide Letters

Volume 20 , Issue 11 , 2013

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Abstract:

Present study dealt with the production, purification and characterization of xylanase from Bacillus subtilis- BS05 grown in submerged fermentation at 37oC for 48h using sugarcane bagasse as a substrate. Xylanase enzyme was purified to homogeneity by ammonium sulphate fractionation followed by sephadex G-100 chromatography. The molecular mass of xylanase enzyme was found to be 23kDa by SDS-PAGE. The optimum pH and temperature of xylanase enzyme was 5 and 50oC with stability range of 5-6 and 30oC -50oC respectively. The enzyme had half life of 1386-1155 minutes at 30oC -50oC respectively. Metal profile of the enzyme showed that Mn2+ (127.4 %) and Fe2+ (115%) were the activators while Hg2+ (14%) and EDTA (19%) were the inhibitors. Kinetic parameters revealed that the enzyme is specific to birchwood xylan with Km , Kcat , Vmax and Kcat/Km value of 1.15 mg/ml, 850 s-1, 117.64 U/mg and 739.13 s-1mg-1.mL respectively.

Keywords: Xylanase, purification, characterization, Bacillus sp.

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Article Details

VOLUME: 20
ISSUE: 11
Year: 2013
Page: [1225 - 1231]
Pages: 7
DOI: 10.2174/09298665113209990007
Price: $65

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