Efficient Method for Agrobacterium Mediated Transformation of Artemisia annua L.
Pravej Alam, Anis Mohammad, M.M. Ahmad, Mather Ali Khan, Mohd. Nadeem, Riyazuddeen Khan, Mohd. Akmal, Seema Ahlawat and M.Z. Abdin
Affiliation: Centre for Transgenic Plant Development, Department of Biotechnology, Faculty of Science, Jamia Hamdard, New Delhi, India 62.
Keywords: Artemisia annua L., T-DNA delivery β−glucuronidase, Genetic transformation.
Artemisinin, a potent antimalarial natural products isolated from aerial parts of Artemisia annua L. Many patents
have been reported that the demand for artemisinin is exponentially increasing year after year due to increased incidences
of drug resistant malaria throughout the world. Leaf explants were used frequently as target tissue to generate
transgenic of Artemisia. annua L. However, obtaining a large number of transgenic lines through out the year is a laborious
and delicate process. To circumvent this, we have developed a highly efficient leaf explant based Agrobacterium mediated
transformation of A. annua L. plant. The gus gene was used as screenable marker to assess and optimize the performance
of T-DNA delivery. The age of explant, kind of bacterial inoculation, suspension duration, infection times and
co-culture conditions were optimized. The co-culture was carried out with Agrobacterium tumefaciens strain EHA105 under
desiccation condition in the dark at 25-28 0C for 2-4 days. Complete analysis of transgene insertion demonstrated that
the optimized method of transformation from leaf explants of A. annua L. was efficient and highly reproducible.
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