Recent Advances in the Syntheses of Nucleoside Triphosphates
Anilkumar R. Kore and Balasubramanian Srinivasan
Affiliation: Life Technologies Corporation, Bioorganic Chemistry Division, 2130 Woodward Street, Austin, TX 78744-1832, USA.
The present review compiles significant advances in different synthetic strategies to obtain various nucleoside triphosphates,
which are indispensable materials for variety of important biological applications such as DNA sequencing, PCR, therapeutic nucleoside
inhibitors of polymerase, to prepare apatamers by in vitro transcription methods, and ingredients in numerous biological assay kits. Modified
nucleoside triphosphate analogs are increasingly evaluated as potential diagnostic and therapeutic agents as well as tools for reengineering
DNA. This review covers literatures after the year 2000. The intent of this review is to summarize the most useful approaches
that have been applied for nucleoside triphosphates syntheses with their advantages, limitations, and generality of methods. The
present review will highlight most practical approaches to access nucleoside triphosphates and also research areas wherein further work
might lead to superior and general approach. It will enable researchers to choose the right method for synthesizing their nucleoside
triphosphates of interest by understanding pros and cons of many available methodologies. It will keep the researchers well informed of
the state-of-the-art in the nucleoside triphosphate syntheses and the pressing needs.
Keywords: Chemical synthesis, enzymatic synthesis, nucleoside triphosphates, phosphorylation, solid supported synthesis.
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