A simple and fast homogeneous fluorescent polarization immunoassay (FPIA) was developed for the
determination of furaltadone and its metabolite 3-amino-5-methylmorpholino-2-oxazolidinone (AMOZ). Monoclonal
antibody with high cross-reactivity to furaltadone and the nitrophenyl derivative of AMOZ (NPAMOZ) were produced
against a novel immunogen and the effects of several synthesized tracers on FPIA sensitivity studied. The proposed FPIA,
using an optimum antibody and tracer pair, had an IC50 of 4.3µg L–1 and limit of detection at 0.6µg L–1 for furaltadone,
and 2.7 µgL–1and0.3µg L–1 for NPAMOZ. Recoveries of furaltadone from animal feeds by FPIA ranged from 79.6 to
87.7%, while recoveries of AMOZ from animal tissues ranged from 72.9 to 83.1%. Good correlation (R>0.99) between
the results of this FPIA and a standard analytical method was obtained. The FPIA does not require separation or washing
steps and the total time required for equilibrium of the antibody-tracer interaction is only 10 min. These results indicated
that the proposed FPIA offers great potential and utility for the high throughput screening of furaltadone residues in
animal feed and its metabolite AMOZ residues in animal tissues.
Keywords: 3-amino-5-methylmorpholino-2-oxazolidinone, fluorescent polarization immunoassay, furaltadone, high
throughput screening, monoclonal antibody, tracer.
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