Title:Rapid Fluorimetric Quantitation of Ibandronate by Coupling Quantum Dots and Multicommutated Flow Injection Analysis
VOLUME: 9 ISSUE: 3
Author(s):A. Ruiz-Medina, L. Molina-Garcia, E.J. Llorent-Martinez, J.L.M. Santos and M.L. Fernandez-de Cordova
Affiliation:Department of Physical and Analytical Chemistry, Faculty of Experimental Sciences, University of Jaen, Campus las Lagunillas, E-23071 Jaen, Spain.
Keywords:Automation, CdTe quantum dots, ibandronate, luminescence, multicommutation, nanoparticle.
Abstract:Ibandronate is a potent nitrogen-containing bisphosphonate with a tertiary amine group, which does not easily
form chromophore derivatives that can be detected by UV light of fluorescence emission. Here, a simple and straightforward
automated multicommutated flow system, making use of water-soluble mercaptopropionic acid-capped CdTe quantum
dots, was implemented for the fluorescence quantitation of ibandronate in pharmaceutical formulations. The developed
approach was based on the analyte ability to establish surface interactions that resulted in quenched nanocrystals
fluorescence intensity, being this effect proportional to the target compound’s concentration. Size and concentration of
quantum dots and pH of the media were some of the most relevant and influencing parameters studied. The proposed
methodology allowed the determination of ibandronate in the range of 20-200 µg mL-1, with good repeatability
(RSD<3%) and a sampling frequency of about 70 samples per hour. The results obtained in the analysis of pharmaceuticals
showed excellent agreement with those provided by the manufacturer.
