Title:Tumor Marker Detection by Aptamer-Functionalized Graphene Oxide
VOLUME: 17 ISSUE: 2
Author(s):Ming-Yu Lin, Yen-Pei Lu, Alexandru M. Grumezescu, Fu H. Ho, Ying-Hsien Kao, Yuh-Shyong Yang and Chih-Hui Yang
Affiliation:Department of Biological Science & Technology, I-Shou University, Taiwan.
Keywords:Aptamers, Biosensors, Functionalization, Graphene oxide, Platelet-derived growth factors, Tumor marker
Abstract:Interaction of the single-stranded DNA (ssDNA) aptamers and proteins was investigated on graphene oxide (GO)-based
biosensors. GO was applied for biological applications because of its unique properties, which include water dispensability, exclusive
selection of ssDNA, and fluorescence-quenching effects. In this study, aptamer-functionalized GO sheets of nanometer-scale thickness
and micrometer-scale dimension were developed. Based on a simple mixed-and-measured strategy, platelet-derived growth factors
(PDGF), the tumor marker of ovarian cancer, triggered aptamers conformation switched and deliberated aptamers from GO surface
according to thermal dynamic analysis. By functionalizing with various aptamers on GO, the strategy could provide designed functions
on GO-based biosensors, bioreactors, and other devices.
