New Informatics and Automated Infrastructure to Accelerate New Leads Discovery by High Throughput Screening (HTS)
Preston S. Donover, Marlin Yohn, Matthew Sim, Andrew Wright, Sandesh Gowda, Chip Allee, Amanda R. Schabdach and Melvin Reichman
Affiliation: CGC, Inc. (Lankenau Institute for Medical Research Chemical Genomics Center), 100 Lancaster Avenue, Wynnewood, PA 19096, USA.
Keywords: Automated sample retrieval system (ASRS), bio-repository, drug discovery, HTS, orthogonal pooling, sample
management, sample store
The Lankenau Institute for Medical Research Chemical Genomics Center, Inc. has developed a new (patents
issued and pending) Nanotube Automated Repository System (NARS) for dynamic storage of millions of ‘single-shot’
samples stored in a new monolithic microtiter-storage tube plate of our own design we call ‘nanotubes.’ We have
integrated the NARS with customized software to efficiently access up to 10,000,000 samples stored continuously frozen
(–20°C) in a dehumidified enclosure and sealed in a new microtiter NARS plate that is SBS compliant. Additional
software was developed to analyze HTS data from orthogonally pooled compound libraries. Following ‘de-convolution’
of pooled HTS data, the software designates confirmatory retest samples to be ‘cherry-picked’ using the NARS. The
application of a new, fully-integrated infrastructure for new leads discovery is described in detail. Other applications for
our technologies and new infrastructure are discussed.
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