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Combinatorial Chemistry & High Throughput Screening
ISSN (Print): 1386-2073
ISSN (Online): 1875-5402
VOLUME: 16
ISSUE: 3
DOI: 10.2174/1386207311316030003      Price:  $58









New Informatics and Automated Infrastructure to Accelerate New Leads Discovery by High Throughput Screening (HTS)

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Author(s): Preston S. Donover, Marlin Yohn, Matthew Sim, Andrew Wright, Sandesh Gowda, Chip Allee, Amanda R. Schabdach and Melvin Reichman
Pages 180-188 (9)
Abstract:
The Lankenau Institute for Medical Research Chemical Genomics Center, Inc. has developed a new (patents issued and pending) Nanotube Automated Repository System (NARS) for dynamic storage of millions of ‘single-shot’ samples stored in a new monolithic microtiter-storage tube plate of our own design we call ‘nanotubes.’ We have integrated the NARS with customized software to efficiently access up to 10,000,000 samples stored continuously frozen (–20°C) in a dehumidified enclosure and sealed in a new microtiter NARS plate that is SBS compliant. Additional software was developed to analyze HTS data from orthogonally pooled compound libraries. Following ‘de-convolution’ of pooled HTS data, the software designates confirmatory retest samples to be ‘cherry-picked’ using the NARS. The application of a new, fully-integrated infrastructure for new leads discovery is described in detail. Other applications for our technologies and new infrastructure are discussed.
Keywords:
Automated sample retrieval system (ASRS), bio-repository, drug discovery, HTS, orthogonal pooling, sample management, sample store
Affiliation:
CGC, Inc. (Lankenau Institute for Medical Research Chemical Genomics Center), 100 Lancaster Avenue, Wynnewood, PA 19096, USA.