Molecular Signature of Human amniotic Fluid Stem Cells During Fetal Development
Paolo De Coppi,
Pascale V. Guillot.
Mid-gestation c-KIT+ amniotic fluid stem cells (AFSC) have an intermediate phenotype between embryonic
and adult stem cells and are easy to reprogram to pluripotency. We previously showed that 1st trimester AFSC can be reprogrammed
to functional pluripotency in a transgene-free approach. Despite both parental populations sharing a common
phenotype, expressing CD29, CD44, CD73, CD90, CD105, SSEA4 and OCT4, 2nd trimester AFSC, contrary to 1st trimester
cells, do not express NANOG, SSEA3, TRA-1-60 and TRA-1-81, and have slower growth kinetics. Here, we used the
Illumina Beadstudio microarray platform to analyse the transcriptome of 1st and 2nd trimester AFSC and show a unique 1st
trimester AFSC-specific gene expression signature consisting of 366 genes and a larger set of 603 genes common with
hESC compared to 496 genes overlapping between 2nd trimester AFSC and hESC. We conclude that both populations are
related but distinct to each other as well as to hESC.
Keywords: Amniotic fluid, amniotic fluid stem cells, gene expression, human embryonic stem cells, stem cells, transcriptome,
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