Nanoparticulate Carrier Mediated Intranasal Delivery of Insulin for the Restoration of Memory Signaling in Alzheimer’s Disease
Rakesh Kumar Tekade,
Narendra Kumar Jain.
The present study was aimed to determine the therapeutic potential of novel carriers to deliver insulin into brain, by passing
the BBB. PLGA nanoparticles and PEGylated PLGA nanoparticles were prepared by double emulsification method. PEG-PLGA copolymer
was synthesized and characterized by FTIR, NMR and Mass spectroscopies. The release profiles of drug in various formulations
were studied in PBS (pH 7.4). Results showed more sustained release of drug with Tween-80 based formulation in comparison with
Tween-20 and PVA based formulations. A more sustained and extended release was observed upon chitosan coating of PEG-PLGA
nanoparticles. Blood glucose level monitoring suggested that glucose level was not decreased significally in the peripheral region
(p>0.05), when chitosan coated insulin loaded PEGylated nanoparticle was administered by intranasal route. This outcome in particular
along with expected mucoadhesive and targeted benefit associated with chitosan based formulation drove us to conclude this formulation
to be working best for the undertaken brain delivery issue.
Keywords: Co-polymer nanoparticle, insulin, Alzheimer, biodistribution study, restoration of memory signaling
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