Development and Characterization of Lanthanides Doped Hydroxyapatite Composites for Bone Tissue Application
Pp. 87-115 (29)
João Coelho, Sooraj H. Nandyala, Pedro S. Gomes, Mónica P. Garcia, Maria A. Lopes, Maria H. Fernandes and José D. Santos
This work reports the preparation and characterization of newly developed
10CaF2-10Na2CO3-15CaO-59P205-5Si02 glasses, doped with lanthanides, in this case
cerium and lanthanum oxide (10CaF2-10Na2CO3-15CaO-59P205-1CeO2-5SiO2 and
10CaF2-10Na2CO3-15CaO-59P205-1La2O3-5SiO2, respectively). The structure and
morphology of the developed glasses have been investigated by Raman and FTIR
spectroscopy. Scanning electron microscopy with an energy dispersive analyzer and X-ray
mapping was used to assess the morphological properties of the glasses. Glass-ceramic
composites, for bone tissue applications, were obtained by the mixture of 2.5wt% of each
glass with 97.5wt% of hydroxyapatite. These were also analyzed by means of XRD and
SEM. Composites were biologically evaluated with human osteoblastic-like cells.
Lanthanide doped-hydroxyapatite composites revealed an improved biological behaviour,
regarding cell adhesion and proliferation, compared to hydroxyapatite and undoped glasshydroxyapatite
composites. Lanthanide doped composites reported an adequate
biocompatibility, further enhancing the cell adhesion and proliferation, behaviour that
indicates a prospective application in bone tissue engineering.
Lanthanide, glass-hydroxyapatite composites, cell culture,
biocompatibility, bone tissue, biological characterization, biocompatibility, bone tissue
engineering, cytocompatibility, cell culture, cerium oxide doped HA composites, doped
glasses, glass-hydroxyapatite composites, hydroxyapatite, in vitro testing, Lanthanum
oxide doped HA composites, materials characterization, osteoblasts.
FCUP/INESC Porto/Department of Physics, Faculty of Sciences, University of Porto, Rua do Campo Alegre, 687, 4169-007 Porto, Portugal