Effect of Fibrin-binding Synthetic Oligopeptide on the Healing of Full-thickness Skin Wounds in Streptozotocin-induced Diabetic Rats

Author(s): Jae-Eun Chung , Yun-Jeong Kim , Yoon-Jeong Park , Ki-Tae Koo , Yang-Jo Seol , Yong-Moo Lee , In-Chul Rhyu , Young Ku .

Journal Name: Current Pharmaceutical Design

Volume 19 , Issue 7 , 2013

Abstract:

The aim of this study was to investigate whether topical application of fibrin-binding oligopeptides derived from FN promotes wound healing in streptozotocin (STZ)-induced diabetic rats.

Oligopeptides including fibrin-binding sequences (FF3: CFDKYTGNTYRV, FF5 : CTSRNRCNDQ) of FN repeats were synthesized. Each peptide was loaded in 15 x 15 mm fibrous alginate dressings, and the release kinetics of the peptides was evaluated using trinitrobenzene sulfonic acid for 336 hours.

Two full-thickness cutaneous wounds were prepared on the dorsal skin of each 75 diabetes induced rats. Each wound was divided into FF3-loaded alginate dressing group, FF5-loaded alginate dressing group, alginate dressing group and negative control group. Animals were sacrificed at day 0,3,7 and 14. The wound closure rate, inflammation degree, expression of TGF-β1 and hydroxyproline contents were evaluated.

Both FF3 and FF5 peptides were released rapidly within the first 24 hours. FF3-loaded dressing treated wounds closed significantly faster than other wounds at day 3. And at day 14, FF3- & FF5- loaded dressing treated wounds demonstrated less inflammatory cells infiltration than alginate dressing treated and negative group wounds. TGF-β1 positive cells were more abundant in FF3-, FF5-treated alginate dressing treated wound at day 3 and 14. At last, the hyrdroxyproline contents in the FF3, FF5 group were higher at day 7 and day 14.

Topical application of fibrin-binding domain synthetic oligopeptides from FN resulted in acceleration of full-thickness cutaneous wound healing in diabetic rats.

Keywords: Fibronectin, fibrin-binding domain, diabetes mellitus, full-thickness cutaneous wound, wound healing, oligopeptides, fibrous alginate dressings, FF5 peptides, TGF-β1, diabetic rats

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Article Details

VOLUME: 19
ISSUE: 7
Year: 2013
Page: [1321 - 1328]
Pages: 8
DOI: 10.2174/138161213804805676
Price: $58

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