The N-Acetylcysteine-Insensitive Acetic Acid-Induced Yeast Programmed Cell Death Occurs Without Macroautophagy

Author(s): Lucia Antonacci, Nicoletta Guaragnella, Masa Zdralevic, Salvatore Passarella, Ersilia Marra, Sergio Giannattasio.

Journal Name: Current Pharmaceutical Biotechnology

Volume 13 , Issue 15 , 2012

Abstract:

Programmed cell death can occur through two separate pathways caused by treatment of Saccharomyces cerevisiae with acetic acid (AA-PCD), which differ from one another essentially with respect to their sensitivity to Nacetylcysteine (NAC) and to the role played by cytochrome c and metacaspase YCA1. Moreover, yeast can also undergo macroautophagy which occurs in NAC-insensitive manner. In order to gain some insight into the relationship between AA-PCD and macroautophagy use was made of WT and knock-out cells lacking YCA1 and/or cytochrome c. We show that i. macroautophagy is modulated by YCA1 and by cytochrome c in a negative and positive manner, respectively, ii. the NAC-insensitive AA-PCD and macroautophagy differ from one another and iii. NAC-insensitive AA-PCD pathway takes place essentially without macroautophagy, even if the shift of extracellular pH to acidic values required for AA-PCD to occur leads itself to increased or decreased macroautophagy in YCA1 or cytochrome c-lacking cells.

Keywords: Cytochrome c, macroautophagy, N-acetylcysteine, programmed cell death, Saccharomyces cerevisiae, YCA1, c-lacking cells, autophagic cell death, proteolytic activities, mitochondrial function

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Article Details

VOLUME: 13
ISSUE: 15
Year: 2012
Page: [2705 - 2711]
Pages: 7
DOI: 10.2174/138920112804724819
Price: $58

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