Fluorescence Imaging of Mitochondria in Living Cells Using a Novel Fluorene Derivative with a Large Two-Photon Absorption Cross-Section

Author(s): Seiji Tani , Kenta Nakagawa , Takuya Honda , Hiromasa Saito , Yasutaka Suzuki , Jun Kawamata , Makiyo Uchida , Akira Sasaki , Masataka Kinjo .

Journal Name: Current Pharmaceutical Biotechnology

Volume 13 , Issue 14 , 2012

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Abstract:

We have examined optical properties of a fluorene derivative with two positively charged substituents, 1,1’- diethyl-4,4’-(9,9-diethyl-2,7-fluorenediyl-2,1-ethanediyl)dipyridinium perchlorate (1), in water. The photoluminescence quantum yield of 1 was relatively high (35%) for use as a fluorescent probe in water. We also examined two-photon absorption (TPA) properties of 1 in methanol. The maximum value of the TPA cross-section (730 GM at 750 nm, 1 GM = 10-50 cm4 s photon-1 molecule-1) was larger than that for most two-photon-excited fluorescent dyes including a classical mitochondria-selective fluorescent dye rhodamine 123. Preliminary fluorescence imaging experiments of the mitochondria in living Paramecium caudatum and HeLa cells were carried out with 1. Bright green fluorescence was observed from the mitochondria in both living cells loaded 1 without toxicity effects. These our results indicate that water-soluble fluorene derivative 1 is a promising candidate as a two-photon-excited fluorescence probe for mitochondria in living cells.

Keywords: Fluorene Derivative, Fluorescence Imaging, HeLa Cells, Mitochondria, Mitochondria-Selective Fluorescent probe, Paramecium caudatum, Two-photon absorption, fluorene derivative, photoluminescence quantum, Preliminary fluorescence imaging, eukaryotic cells, Bright green fluorescence, cytotoxic effects, yeast cells

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Article Details

VOLUME: 13
ISSUE: 14
Year: 2012
Page: [2649 - 2654]
Pages: 6
DOI: 10.2174/138920101314151120122918
Price: $58

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