In many signaling pathways, various factors have been isolated by molecular genetic studies and wholegenome
analysis. Understanding the interactions among these factors is crucial to understanding the signaling process as a
whole. Recent studies on auxin signaling in the regulation of plant growth and development have discovered primary factors
interacting with each other, and elucidated a very simple pathway modulating gene expression in response to auxin.
However, these studies of auxin-signaling led to the question of how such a simple pathway generates multiple types of
regulation in various processes of different cells throughout the life of a plant. Here, we provide an overview of recent
progress in auxin biology focusing on protein-protein interactions in the signal transduction pathway and discuss various
possibilities and approaches to resolve the issue.
Keywords: Auxin, signal transduction, protein-protein interaction, growth regulation, plant hormone, signaling pathways, molecular genetic studies, various factors, protein-protein interactions, auxin signaling, molecules, fluorescence cross-correlation spectroscopy (FCCS), interacting proteins, co-immunoprecipitation
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