Image Analysis of Colocalization of Nuclear DNA and GFP Labelled HIF-1α in Stable Transformants

Author(s): Takehito Goto , Michihiko Sato , Eiji Takahashi , Yasutomo Nomura .

Journal Name: Current Pharmaceutical Biotechnology

Volume 13 , Issue 14 , 2012

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Abstract:

HIF-1α is regarded as a target for drug development in several diseases such as cancer. For high throughput screening of HIF-1α-targeted drug, we need to examine the activity quantitatively. In the present study, we proposed a method where stable expression system of HIF-1α was combined with image correlation analysis. When the stable transformants were labeled with DRAQ5, we could detect Co2+-induced nuclear translocation by the use of cross-correlation analysis of the dual labeling images. In the case of high throughput screening for HIF-1α-targeted drug, we should use Pearson’s correlation coefficient to judge nuclear translocation.

Keywords: HIF-1α, GFP, stable transformant, colocalization, cobalt, drug development, cancer, DRAQ5, dual labeling images, nuclear translocation, hypoxia response element (HRE), vessel, glycolytic enzymes, hypoxic

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Article Details

VOLUME: 13
ISSUE: 14
Year: 2012
Page: [2547 - 2550]
Pages: 4
DOI: 10.2174/138920101314151120122519
Price: $58

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