HIF-1α is regarded as a target for drug development in several diseases such as cancer. For high throughput
screening of HIF-1α-targeted drug, we need to examine the activity quantitatively. In the present study, we proposed a
method where stable expression system of HIF-1α was combined with image correlation analysis. When the stable transformants
were labeled with DRAQ5, we could detect Co2+-induced nuclear translocation by the use of cross-correlation
analysis of the dual labeling images. In the case of high throughput screening for HIF-1α-targeted drug, we should use
Pearson’s correlation coefficient to judge nuclear translocation.
Keywords: HIF-1α, GFP, stable transformant, colocalization, cobalt, drug development, cancer, DRAQ5, dual labeling images, nuclear translocation, hypoxia response element (HRE), vessel, glycolytic enzymes, hypoxic
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