Proteolysis is doubtlessly the most widespread mechanism of biological regulation. By controlling protein synthesis, turnover
and activity, it is involved in fundamental physiological processes including apoptosis, cell differentiation, growth and signaling, fertilization,
immune response, blood coagulation and digestion. Yet, uncontrolled proteolysis can be harmful for organisms, causing - amongst
others - such diseases as cancer, emphysema, inflammation, and neurodegenerative, immunological, and cardiovascular disorders. This
paper briefly describes recent advances in the development of methodological design to follow up protease activity. Novel methods of
protease sensing are described and evaluated. A variety of fluorescent reporter molecules including nanoparticles, and rare metal chelates
are also characterized.