Drug Metabolism Letters

Zhiyang Zhao
Amgen
Cambridge, MA
USA

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The Pharmacokinetics and Metabolism Study of Sodium 7,4' -oxo-acetic Acid Daidzein in Rat

Author(s): Shujun Wang, Ruixue Gao, Kun Xu, Xiaojun Yi, Wei Hou, Chengyu Liu, Fei Cheng.

Abstract:

Object: To Study the pharmacokinetics and metabolism of Sodium 7,4' -oxo-acetic acid daidzein in rat.

Method: Sodium 7,4' -oxo-acetic acid daidzein was determined by reversed-phase HPLC (column: German CenturySIL BDS C18 5μm silica, 200mm×4.6mm i.d; fluid: methanol-water-85% phosphoric acid(57:43:0.05, v/v/v)), with sodium benzoic acid as an internal standard. Biological samples were extracted with acetonitrile.

Results: The calibration curve was linear over the range of 1.0-1000μg/ml in rat plasma, urine and feces. The average extraction recoveries were 73.3% (plasma), 73.9% (urine) and 83.7% (feces) respectively and the intra-day and inter-day precisions were less than 8.34%. The assay was applied to the analysis of samples from a pharmacokinetic study. The absolute bioavailability of oral administration was 3.07%. DZ I original compound determined in 6h urine was 32.32%, in 24h urine was 32.71%, in 24h feces was 22.96%.

Keywords: DZ I, pharmacokinetics, metabolism, rat, plasma, urine, feces

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Article Details

VOLUME: 6
ISSUE: 2
Year: 2012
Page: [145 - 150]
Pages: 6
DOI: 10.2174/1872312811206020145
Price: $58