Idebenone Treatment Mediates the Effect of Menadione Oxidative Stress Damage in Saccharomyces cerevisiae

Title:Idebenone Treatment Mediates the Effect of Menadione Oxidative Stress Damage in Saccharomyces cerevisiae

Volume: 6 Issue: 2

Author(s): Oliver Gamondi, Sebastian Chapela, Ines Nievas, Isabel Burgos, Manuel Alonso and Carlos Stella

Affiliation:

Keywords: Yeast, idebenone, oxidative damage

Abstract: We investigated the damage caused by oxidative stress using the yeast Saccharomyces cerevisiae as a model biological system. After inducing oxidative stress with menadione, we were able to evaluate the extent of cellular oxidative stress by utilizing 2´,7´-dichlorofluorescein diacetate (DCFH-DA) as a marker of the presence of reactive oxygen species. Cells were grown on different carbon sources in order to compare fermentative and oxidative metabolism. Under these conditions we evaluated the effectiveness of idebenone (2,3-dimethoxy-5-methyl-6-(10- hydroxydecyl)-1,4-benzoquinone) as a molecule that could relieve menadione-induced growth inhibition in Saccharomyces cerevisiae.

Cite this article as:

Gamondi Oliver, Chapela Sebastian, Nievas Ines, Burgos Isabel, Alonso Manuel and Stella Carlos, Idebenone Treatment Mediates the Effect of Menadione Oxidative Stress Damage in Saccharomyces cerevisiae, Drug Metabolism Letters 2012; 6 (2) . https://dx.doi.org/10.2174/1872312811206020120