Drug Metabolism Letters

Zhiyang Zhao
Amgen
Cambridge, MA
USA

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Idebenone Treatment Mediates the Effect of Menadione Oxidative Stress Damage in Saccharomyces cerevisiae

Author(s): Oliver Gamondi, Sebastian Chapela, Ines Nievas, Isabel Burgos, Manuel Alonso, Carlos Stella.

Abstract:

We investigated the damage caused by oxidative stress using the yeast Saccharomyces cerevisiae as a model biological system. After inducing oxidative stress with menadione, we were able to evaluate the extent of cellular oxidative stress by utilizing 2´,7´-dichlorofluorescein diacetate (DCFH-DA) as a marker of the presence of reactive oxygen species. Cells were grown on different carbon sources in order to compare fermentative and oxidative metabolism. Under these conditions we evaluated the effectiveness of idebenone (2,3-dimethoxy-5-methyl-6-(10- hydroxydecyl)-1,4-benzoquinone) as a molecule that could relieve menadione-induced growth inhibition in Saccharomyces cerevisiae.

Keywords: Yeast, idebenone, oxidative damage

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Article Details

VOLUME: 6
ISSUE: 2
Year: 2012
Page: [120 - 123]
Pages: 4
DOI: 10.2174/1872312811206020120
Price: $58